Immunofluorescence staining and confocal microscopy was used to determine the trimethylated-histone H3 (Lys9) (H3K9me3) (Millipore, Billerica, MA) (1:1000). The procedures were performed as previously described32–34 (link). The specimens were incubated for 1 h with Alexa Fluor 594 goat anti-rabbit antibody (abcam, Cambridge, UK; 1:400) after incubation of the primary antibody. Images were analysed using an A1 Nikon confocal laser scanning microscope (Nikon, Tokyo, Japan).
Hwang Y.J., Hyeon S.J., Kim Y., Lim S., Lee M.Y., Kim J., Londhe A.M., Gotina L., Kim Y., Pae A.N., Cho Y.S., Seong J., Seo H., Kim Y.K., Choo H., Ryu H, & Min S.J. (2021). Modulation of SETDB1 activity by APQ ameliorates heterochromatin condensation, motor function, and neuropathology in a Huntington’s disease mouse model. Journal of Enzyme Inhibition and Medicinal Chemistry, 36(1), 856-868.
Procedures were performed as previously described (link)
Specimens were incubated for 1 h with Alexa Fluor 594 goat anti-rabbit antibody (abcam, Cambridge, UK; 1:400) after incubation of the primary antibody
Images were analysed using an A1 Nikon confocal laser scanning microscope (Nikon, Tokyo, Japan)
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