Lung Tissue DNA Sequencing Library Prep

Genomic DNA from Esperanza's lung tisse (used also for long-read sequencing, above) was converted into sequencing libraries using the TruSeq DNA PCR-Free LT Library Preparation Kit (Illumina Inc., San Diego, CA) as directed. The shearing was conducted on a Covaris S220 instrument (Covaris Inc., Woburn, MA) with setting to 350-bp fragment size. The same procedure was used to create libraries for parental and unrelated yak samples, except the DNA was prepared from blood using a standard phenol: chloroform extraction as described previously [29 (link)]. Sequencing was performed by 2 × 150 bp paired-end sequencing on a NextSeq500 instrument (Illumina NextSeq 500, RRID:SCR_014983) using High Output Kit v2 (300 cycles) kits.