DNA was extracted from 0.1 ± 0.03 g wet faeces using the QIAamp PowerFecal DNA kit on a QIAcube (Qiagen, Hilden, Germany) as described previously [14 (link)].
DNA was extracted from cultured L. reuteri with the EZ1 DNA Tissue kit (Qiagen). Broth cultures were centrifuged at 3000 rpm for 10 min at 4 °C, and pellets were resuspended in 500 µL buffer G2, while up to 10 colonies were picked from agar plates and pooled in 500 µL G2 buffer. Samples were transferred to glass bead tubes and shaken with a TissueLyser II for 1 min at 30 Hz. Two hundred µL lysate were subjected to automatised DNA extraction using the protocol for purification of DNA from bacterial culture samples on an EZ1 Advanced XL robot (Qiagen).
DNA concentration was measured with Qubit dsDNA HS Assay kits (Thermo Fisher Scientific, Waltham, MA, USA) according to the manufacturer’s instructions, and DNA was stored at −20 °C.
DNA was extracted from cultured L. reuteri with the EZ1 DNA Tissue kit (Qiagen). Broth cultures were centrifuged at 3000 rpm for 10 min at 4 °C, and pellets were resuspended in 500 µL buffer G2, while up to 10 colonies were picked from agar plates and pooled in 500 µL G2 buffer. Samples were transferred to glass bead tubes and shaken with a TissueLyser II for 1 min at 30 Hz. Two hundred µL lysate were subjected to automatised DNA extraction using the protocol for purification of DNA from bacterial culture samples on an EZ1 Advanced XL robot (Qiagen).
DNA concentration was measured with Qubit dsDNA HS Assay kits (Thermo Fisher Scientific, Waltham, MA, USA) according to the manufacturer’s instructions, and DNA was stored at −20 °C.
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