Comprehensive Clinical Biomarker Profiling

Blood samples were collected at the Hainan Provincial People’s Hospital for the measurements of clinical indices, including metabolic parameters (TGs, TC, and FPG), sex hormones (LH, FSH, LH/FSH, E2, PRL, and T) and gut-brain mediators (PYY and ghrelin). The sex hormones were measured on day 3 of the menstrual cycle. The blood biochemical indices were measured on an automatic biochemical analyzer (BS-220; Mindray, China), and the sex hormones were tested using an automated immunoassay system (AIA-1200; Tosoh, Japan). The ghrelin and PYY levels were determined using commercially available enzyme-linked immunosorbent assay (ELISA) kits according to the manufacturer’s instructions (12 (link)). To determine SCFA levels, we used approximately 250 mg of frozen fecal samples. The concentrations of SCFAs were determined using a 1:25 dilution of 500 μl of supernatant. Gas chromatography-mass spectrometry (GC-MS) determinations were performed using a Varian Saturn 2000 GC-MS instrument with an 8200 CX solid-phase microextraction (SPME) autosampler (54 (link)).

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Zhang J., Sun Z., Jiang S., Bai X., Ma C., Peng Q., Chen K., Chang H., Fang T, & Zhang H. (2019). Probiotic Bifidobacterium lactis V9 Regulates the Secretion of Sex Hormones in Polycystic Ovary Syndrome Patients through the Gut-Brain Axis. mSystems, 4(2), e00017-19.

Publication 2019

BS-220 AIA-1200

Blood Brain Dilution Enzyme linked immunosorbent assay Fecal Frozen Gas chromatography mass spectrometry Ghrelin Immunoassay Menstrual cycle Sex hormones Solid phase microextraction

Corresponding Organization :

Other organizations : Hainan University, Inner Mongolia Agricultural University, Haikou City People's Hospital

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

Metabolic parameters (TGs, TC, and FPG)
Sex hormones (LH, FSH, LH/FSH, E2, PRL, and T)
Gut-brain mediators (PYY and ghrelin)
Short-chain fatty acid (SCFA) levels

control variables

Sex hormones were measured on day 3 of the menstrual cycle
Blood biochemical indices were measured on an automatic biochemical analyzer (BS-220; Mindray, China)
Sex hormones were tested using an automated immunoassay system (AIA-1200; Tosoh, Japan)
Ghrelin and PYY levels were determined using commercially available enzyme-linked immunosorbent assay (ELISA) kits
SCFA levels were determined using gas chromatography-mass spectrometry (GC-MS) with a Varian Saturn 2000 GC-MS instrument and an 8200 CX solid-phase microextraction (SPME) autosampler

positive controls

None explicitly mentioned

negative controls

None explicitly mentioned

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