Quantitative gene expression analysis

Analysis of gene expression using qPCR was performed as previously described [49 (link)]. The reactions were performed on ViiA Real-Time PCR System (Applied Biosystems) with Taqman Fast Universal PCR Master Mix (Life Technologies). The primer sets for all the genes was purchased from Life Technologies. The expression level of the genes was calculated using standard ΔΔCt method, with GAPDH as internal control. All reactions were performed with at least 3 replicates.

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Wei Q., Tang Y.J., Voisin V., Sato S., Hirata M., Whetstone H., Han I., Ailles L., Bader G.D., Wunder J, & Alman B.A. (2015). Identification of CD146 as a marker enriched for tumor-propagating capacity reveals targetable pathways in primary human sarcoma. Oncotarget, 6(37), 40283-40294.

Publication 2015

ViiA Real-Time PCR System Taqman Fast Universal PCR Master Mix

Expression genes Gapdh Gene expression analysis Genes Primer

Corresponding Organization : University of Toronto

Other organizations : University Health Network, Princess Margaret Cancer Centre, Mount Sinai Hospital, Lunenfeld-Tanenbaum Research Institute

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

Expression level of the genes

control variables

GAPDH as internal control

controls

Positive control: Not specified
Negative control: Not specified

Annotations

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