PEG–AgNPs were examined using transmission electron microscopy (TEM), as previously reported [8 (link),45 (link)]. In brief, the AgNP suspensions were sonicated for 15 min at room temperature (RT). The PEG–AgNP suspensions were placed on a 200 mesh Formvar/Carbon-coated copper grid and allowed to dry at room temperature for an hour. Thereafter, the grids were analyzed, and several images were captured at various magnifications using a TecnaiTM, (Casoria, Italy) G2 Spirit transmission microscope (FEI Company, Hillsboro, OR, USA).
Regarding the zeta potential analysis of PEG–AgNPs, all samples were diluted to 10% by volume in absolute ethanol, vortexed for 10 min, and then measured for size and zeta potential. The Malvern Zetasizer instrument (Malvern Panalytical, Malvern, UK) and Zetasizer 7.11 software were used for measurement and data processing. All measurements were performed in triplicate and at room temperature.
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