Western Blot Analysis of Signaling Proteins

Western blotting analysis performed as previously described [28 (link), 29 (link)]. The following antibodies were used: S100A8 (1/1000; R&D, MAB4570); S100A9 (1/1000; Santa-Cruz,8114); MST1 (1/1000; Cell Signaling Technology, 3682, Boston, USA); LATS1 (1/1000; Cell Signaling Technology, 3477); pLATS1-T1079 (1/1000; Cell Signaling Technology, 8654); YAP (1/1000; Cell Signaling Technology, 4912); pYAP-S127 (1/1000; Cell Signaling Technology, 13008S); anti-Flag tag (CWBIO, CW0287A, Beijing, China); anti-His tag (MBL, D291–3, NAGOYA, JAPAN). GAPDH (ZSGB-BIO, TA-08, Beijing, China) was used as loading control.

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Li Y., Kong F., Jin C., Hu E., Shao Q., Liu J., He D, & Xiao X. (2019). The expression of S100A8/S100A9 is inducible and regulated by the Hippo/YAP pathway in squamous cell carcinomas. BMC Cancer, 19, 597.

Publication 2019

LATS1 pLATS1-T1079 pYAP-S127 anti-Flag tag GAPDH

Antibodies Gapdh Lats1 Western blotting analysis

Corresponding Organization :

Other organizations : Beijing Normal University

Top 5 similar protocols

Variable analysis

independent variables

Antibodies used: S100A8 (1/1000; R&D, MAB4570)
S100A9 (1/1000; Santa-Cruz,8114)
MST1 (1/1000; Cell Signaling Technology, 3682, Boston, USA)
LATS1 (1/1000; Cell Signaling Technology, 3477)
PLATS1-T1079 (1/1000; Cell Signaling Technology, 8654)
YAP (1/1000; Cell Signaling Technology, 4912)
PYAP-S127 (1/1000; Cell Signaling Technology, 13008S)
Anti-Flag tag (CWBIO, CW0287A, Beijing, China)
Anti-His tag (MBL, D291–3, NAGOYA, JAPAN)

dependent variables

Western blotting analysis

control variables

GAPDH (ZSGB-BIO, TA-08, Beijing, China) used as loading control

controls

No positive or negative controls explicitly mentioned

Annotations

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