Single-cell RNA-seq of Mesenchymal Cells
Corresponding Organization :
Other organizations : University of Massachusetts Chan Medical School, UMass Memorial Medical Center, University of Michigan–Ann Arbor
Variable analysis
- Single-cell library preparation using Chromium Single Cell 3′ GEM Library & Gel Bead Kit v3
- Sequencing outputs processed using CellRanger software
- Reads mapped to human reference genome GRCh38 (Ensembl 93)
- RNA velocity analysis using velocyto v.0.17 commend line tool and velocyto.R v.0.6 R package
- Sequencing performed on the NextSeq 500 system using the NextSeq 500/550 High Output kits v.2.5
- Data analysis performed using Seurat v.4.1.0 within R v.4.0.2 environment
Annotations
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