Luciferase assays for transfections with the CHIKVRep split replication system, CHILuc and LucCHI were performed as previously described [13 (link),26 (link)] with the Dual-Luciferase Reporter Assay (Promega) on a GloMax multi+ plate reader (Promega) (S4 and S5 Figs). The assays were carried out according to manufacturer’s instructions.
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