Chromatin Immunoprecipitation (ChIP) Protocol

ChIP assays were performed using an EZ-CHIP KIT according to the manufacturer's instructions (Millipore, USA). EZH2 antibody was obtained from Abcam. H3 trimethyl Lys 27 antibody was from Millipore. The ChIP primer sequences are listed in Supplementary Table S1. Quantification of immunoprecipitated DNA was performed using qPCR with SYBR Green Mix (Takara). ChIP data is presented as a percentage relative to the input DNA using the equation 2^{[Input Ct- Target Ct]} ×0.1 × 100 [19 (link)].

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Chen W.M., Huang M.D., Sun D.P., Kong R., Xu T.P., Xia R., Zhang E.B, & Shu Y.Q. (2016). Long intergenic non-coding RNA 00152 promotes tumor cell cycle progression by binding to EZH2 and repressing p15 and p21 in gastric cancer. Oncotarget, 7(9), 9773-9787.

Publication 2016

EZ-CHIP KIT EZH2 antibody H3 trimethyl Lys 27 antibody SYBR Green Mix

Antibody Chip Chip assays Ezh2 Primer Sybr green

Corresponding Organization :

Other organizations : Nanjing Medical University, Huaian First People’s Hospital, Jiangsu Province Hospital

Top 5 similar protocols

Variable analysis

independent variables

EZH2 antibody
H3 trimethyl Lys 27 antibody

dependent variables

Quantification of immunoprecipitated DNA

control variables

ChIP assays performed using an EZ-CHIP KIT according to the manufacturer's instructions
ChIP primer sequences listed in Supplementary Table S1
Quantification of immunoprecipitated DNA performed using qPCR with SYBR Green Mix (Takara)

controls

Positive control: Not specified
Negative control: Not specified

Annotations

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