Cell apoptosis were evaluated by flow cytometry analysis utilizing Annexin V-FITC/PI apoptosis detection kit (BB-4101, Bestbio, China). In short, OGCs were harvested and washed, and then stained with Annexin V-FITC and PI in the darkroom, followed by flow cytometry, and analyzed using Flow Jo 7.6 software.
Cell proliferation was assessed by EDU incorporation assay with an EDU imaging kit (C10310-1, RiboBio, China), as described in our previous study [9 (link)]. In short, EDU reagent was added to OGCs in each well for 2 h incubation, followed by fixation, permeabilization, EDU staining, and DAPI counterstaining. The results of proliferation rate were analyzed by the percentage of EDU-stained OGCs to DAPI-stained OGCs.
Cell proliferation was assessed by EDU incorporation assay with an EDU imaging kit (C10310-1, RiboBio, China), as described in our previous study [9 (link)]. In short, EDU reagent was added to OGCs in each well for 2 h incubation, followed by fixation, permeabilization, EDU staining, and DAPI counterstaining. The results of proliferation rate were analyzed by the percentage of EDU-stained OGCs to DAPI-stained OGCs.
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