Isolation of Eutypa lata Secondary Metabolites

We obtained the E. lata secondary metabolites eutypine, eutypinol, siccayne and eulatinol by fermentation of Eutypa lata, strain IBWF E16121 in 20 liters of BAF medium as described in Guan et al. (2020) [20 (link)]. We dissolved the fungal metabolites in 100% methanol to a stock of 10 mM, diluting to a working concentration of 10 μM, if not specified otherwise. The chemical homologues of the fungal metabolites, 4-HBAL (4-hydroxybenzyl aldehyde), 4-HBA (4-hydroxybenzyl alcohol), 4-methoxyphenol and hydroquinone were all purchased from Sigma-Aldrich (Merck, Darmstadt, Germany) and prepared in 2% methanol to stocks of 10 mM stocks and used at a final concentration of 50 μM, if not stated otherwise.