After LDLR–/– mice were fasted for 6 h, blood samples were collected from heart. The samples were centrifuged at 500 g for 20 min at 4°C to separate plasma. Plasma TC and TG contents, HDL-C and LDL-C levels were detected with kits from BioSino Bio-technology and Science Inc. (Beijing, China). Plasma AST, ALT and ICAM-1 levels were measured by enzyme-linked immunosorbent assay using an insulin ultrasensitive ELISA kit (R&D, Minneapolis, MN, United States), according the instruction of the manufacture. The color absorbance at 450 nm was measured using a Bio-Rad microplate reader.
Plasma cytokines were determined by BD Cytometric Bead Array with a flow cytometer (FACS Calibur, BD, Franklin Lakes, NJ, United States) at the end of experiments as described previously (Zhang et al., 2014 (link); Mao et al., 2015 (link)). The following cytokines were determined in this study using their corresponding antibody: IL-1α, IL-1β, IL-6, IL-10, TNF-α, MCP-1, and MIP-1 α (R&D Systems, Minneapolis, MN, United States).
Plasma cytokines were determined by BD Cytometric Bead Array with a flow cytometer (FACS Calibur, BD, Franklin Lakes, NJ, United States) at the end of experiments as described previously (Zhang et al., 2014 (link); Mao et al., 2015 (link)). The following cytokines were determined in this study using their corresponding antibody: IL-1α, IL-1β, IL-6, IL-10, TNF-α, MCP-1, and MIP-1 α (R&D Systems, Minneapolis, MN, United States).
Free full text: Click here
