We used the following antibodies: rabbit anti-GFP (1:2000; Molecular Probes), chicken anti-GFP (1:2000; Abcam), mouse anti-parvalbumin (1:2000; Sigma-Aldrich), rabbit anti-GFAP (1:20 000; Dako), rabbit anti-Iba1 (1:1000; Wako), rat anti-somatostatin (1:5000; Millipore), mouse anti-GABA (1:4000; Sigma-Aldrich), and mouse anti-NeuN (1:2000; Chemicon).
Immunohistochemistry was performed as described previously (Braz et al., 2012 (link)). Sections were viewed with a Nikon Eclipse fluorescence microscope, and images were collected with a Zeiss camera (Axiocam). High-resolution confocal images taken on a Zeiss confocal confirmed that the immunoreactivity was cytoplasmic (0.8 µm optical sections). Brightness and contrast were adjusted using ImageJ (Version 1.49b).
Immunohistochemistry was performed as described previously (Braz et al., 2012 (link)). Sections were viewed with a Nikon Eclipse fluorescence microscope, and images were collected with a Zeiss camera (Axiocam). High-resolution confocal images taken on a Zeiss confocal confirmed that the immunoreactivity was cytoplasmic (0.8 µm optical sections). Brightness and contrast were adjusted using ImageJ (Version 1.49b).
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