Cell Culture Conditions for Various Cell Lines

U2OS, HeLa, HeLa-S, 293T cells, and MEFs were cultured in DMEM (Invitrogen), supplemented with 10% FBS (Atlanta Biologicals) and 100 U/ml penicillin-streptomycin (Gibco) at 37°C and 5% CO₂. BRCA1 mutant ovarian cancer cell line UWB1.289 (UWB1) and its complemented derivative expressing WT BRCA1 (UWB1 + BRCA1; DelloRusso et al., 2007 (link)) cells were grown in 50% RPMI medium, 50% mammary epithelial cell growth medium bullet kit (Lonza CC-3150) completed with 3% FBS, 100 U/ml penicillin, and 100 µg/ml streptomycin at 37°C in 5% CO₂. Human triple-negative breast cancer cells HCC 1937 (Tassone et al., 2003 (link), 2005 (link)) were cultured in RPMI complemented with 10% FBS, 100 U/ml penicillin, and 100 µg/ml streptomycin at 37°C in 5% CO₂. Sf9 cells were grown in suspension in Sf-900 II serum-free medium (Gibco), supplemented with 100 U/ml penicillin-streptomycin (Gibco) at 27°C.