Genomic DNA for SNP-DArT (DArTseq) and silico-DArT genotyping was isolated from 329 plants of the G38A mapping population and the two parental lines using the Mag-Bind Plant DNA 96 Kit (Omega Bio-Tek, USA) in accordance with the manufacturer’s instructions. One hundred milligrams of ground leaf tissue was used for each analyzed plant. The isolated DNA was dissolved in 100 μl elution buffer. The DNA yield and purity were estimated using a spectrophotometer (NanoDrop 2000, Thermo Fisher Scientific, USA) and electrophoresed in 1% agarose gel stained with SimplySafe (Eurx, Poland). The DNA concentration was adjusted to 100 ng and then samples were sent to the Diversity Arrays Technology Pty Ltd. (Bruce, Australia), where genotyping was performed employing the DArTseq 1.0 technology developed by Cruz et al. [29 (link)] (Table S2 and S3 ).
Marker annotation was performed by mapping the sequences and chromosome positions to the Lo7 rye genome [15 (link)]. All gene names mentioned in the text are taken from Rabanus-Wallace et al. [15 (link)].
Marker annotation was performed by mapping the sequences and chromosome positions to the Lo7 rye genome [15 (link)]. All gene names mentioned in the text are taken from Rabanus-Wallace et al. [15 (link)].
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