Murine Gene Expression Analysis by qPCR

The gene expression analysis was performed using an ABI 7500 Real-Time PCR (Life Technologies, Darmstadt, Germany). Genexpression analysis for the murine (ms) genes IL-6, TNF-α, CXCL1, TLR2, TLR4, mFPR1, mFPR2 and mFPR3 were performed. The Primers for mFPR1 and mFPR2 were published previously [26] (link). Murine qPCR Primers were designed using the Primer Express 3.0 Software provided by Life Technologies (Darmstadt, Germany). Specific PCR products were detected by Sybr-Green and changes in gene expression were analysed by the ΔΔCT-calculation. GAPDH was used as a housekeeping gene (Table 1).

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Giebeler A., Streetz K.L., Soehnlein O., Neumann U., Wang J.M, & Brandenburg L.O. (2014). Deficiency of Formyl Peptide Receptor 1 and 2 Is Associated with Increased Inflammation and Enhanced Liver Injury after LPS-Stimulation. PLoS ONE, 9(6), e100522.

Publication 2014

ABI 7500 Real-Time PCR Primer Express 3.0 Software

Cxcl1 Gapdh Gene expression Gene expression analysis Genes Housekeeping gene Murine Primer Real time pcr Sybr green Tlr2 Tnf α

Corresponding Organization :

Other organizations : RWTH Aachen University, Institute for Sports Medicine, Ludwig-Maximilians-Universität München, National Cancer Institute, Center for Cancer Research

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

Gene expression of murine (ms) genes IL-6, TNF-α, CXCL1, TLR2, TLR4, mFPR1, mFPR2 and mFPR3

control variables

GAPDH used as a housekeeping gene

controls

No positive or negative controls were explicitly mentioned

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