Protocol detail

Isolation and Analysis of Colon Leukocytes

Protocol full text hidden due to copyright restrictions

However, the full text is available at the following link: Access Free Full Text.

Sinha S.R., Haileselassie Y., Nguyen L.P., Tropini C., Wang M., Becker L.S., Sim D., Jarr K., Spear E.T., Singh G., Namkoong H., Bittinger K., Fischbach M.A., Sonnenburg J.L, & Habtezion A. (2020). Dysbiosis-induced Secondary Bile Acid Deficiency Promotes Intestinal Inflammation. Cell host & microbe, 27(4), 659-670.e5.

Publication 2020

collagenase IV Zombie Aqua TNFα-Alexa 488 FlowJo software

Antibodies Brefeldin a Cells Collagenase Colon Cytokines Density gradient centrifugation Dyes Edta Flow cytometry Hbss Il17 Intracellular Ionomycin Leukocytes Percoll Stain Tissues Tnfα

Corresponding Organization : Stanford University

Other organizations : Children's Hospital of Philadelphia, Chan Zuckerberg Initiative (United States)

Top 5 similar protocols

Variable analysis

Dependent Variables detection not available due to copyright restrictions.
Unable to provide accurate variables.

Annotations

Annotations not available due to copyright restrictions

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!