Quantitative RT-PCR Analysis of Key Targets

Total RNAs were extracted using TRIzol reagent and reverse-transcribed, as described [29 (link),41 (link)]. Quantitative real-time RT-PCR analysis (qRT-PCR) was performed and normalized with the internal control β-actin (ACTB) using commercial ready-to-use primers/probe mixes (Assays on Demand Products, Applied Biosystems): CD147 (assay Hs 00936295_m1), MCT4 (assay Hs00358829_m1), ACTB (assay Hs 9999903_m1) and ACE2 (assays Hs 01085331_m1 and Hs 01085333_m1) [29 (link),41 (link)]. The ABI PRISM 7700 DNA Sequence Detection System (Applied Biosystems, Foster City, CA, USA) was used [29 (link),41 (link)].

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Spinello I., Saulle E., Quaranta M.T., Pelosi E., Castelli G., Cerio A., Pasquini L., Morsilli O., Dupuis M.L, & Labbaye C. (2024). AC-73 and Syrosingopine Inhibit SARS-CoV-2 Entry into Megakaryocytes by Targeting CD147 and MCT4. Viruses, 16(1), 82.

Publication 2024

ABI PRISM 7700 DNA Sequence Detection System

Corresponding Organization : Istituto Superiore di Sanità

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Variable analysis

independent variables

Not explicitly mentioned

dependent variables

Relative expression levels of CD147, MCT4, ACE2

control variables

ACTB (β-actin) as internal control for normalization of qRT-PCR

controls

Positive control: Not specified
Negative control: Not specified

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