Multimodal Imaging of Vascular and Cell Structures

The vessel
sample was fixed with 4% of paraformaldehyde in PBS overnight at 4
°C and incubated with a 1:50 diluted Alexa Fluor 488 antihuman
CD31 antibody (BioLegend) and 50 μg/mL of DAPI overnight at
4 °C. The fibroblast-laden beads were fixed and incubated with
a human α-smooth muscle actin (α-SMA) Alexa Fluor 594-conjugated
antibody (R&D System).^{71 (link)} The HCT-116-laden
beads were fixed and incubated with an antihuman CD326 (EpCAM) antibody
(BioLegend).^{72 (link)} The vessel network sample
was fixed and incubated with an Alexa Fluor 488 antimouse CD31 (PECAM)
antibody (BioLegend).^{73 (link)} All of the fluorescent
images and confocal scanning were acquired with a Nikon C2+ laser
scanning confocal microscope in the Health Research Hub Center, Lehigh
University.

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Wu Y., Zhao Y., Zhou Y., Islam K, & Liu Y. (2023). Microfluidic Droplet-Assisted Fabrication of Vessel-Supported Tumors for Preclinical Drug Discovery. ACS Applied Materials & Interfaces, 15(12), 15152-15161.

Publication 2023

Alexa Fluor 488 antihumanCD31 antibody antihuman CD326 (EpCAM) antibody Nikon C2+ laserscanning confocal microscope

Alexa 594 Alexa fluor 488 Antibody Confocal microscope Dapi Epcam Fibroblast Human α smooth muscle actin Paraformaldehyde Vessel

Corresponding Organization : Lehigh University

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Variable analysis

independent variables

Vessel sample fixed with 4% paraformaldehyde in PBS overnight at 4 °C
Vessel sample incubated with 1:50 diluted Alexa Fluor 488 anti-human CD31 antibody and 50 μg/mL of DAPI overnight at 4 °C
Fibroblast-laden beads fixed and incubated with human α-smooth muscle actin (α-SMA) Alexa Fluor 594-conjugated antibody
HCT-116-laden beads fixed and incubated with anti-human CD326 (EpCAM) antibody
Vessel network sample fixed and incubated with Alexa Fluor 488 anti-mouse CD31 (PECAM) antibody

dependent variables

Fluorescent images and confocal scanning acquired with Nikon C2+ laser scanning confocal microscope

control variables

Incubation conditions (overnight at 4 °C)

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