A total of 37 formalin-fixed, paraffin-embedded tissues were obtained from patients diagnosed with SCLC by bronchofiberscopy or biopsy between January 2012 and March 2015. All patients received care and follow-up in our hospital. Informed consent was obtained under the protocol approved by our hospital's ethics committee. The clinical characteristics are summarized in Table 1 .
The endogenous peroxidase activity was blocked by soaking the deparaffinized specimens in 3.3% H2O2. The specimens were then incubated with primary antibodies (MST2, 1 : 200, ab87322, abcam; pMST2 (phosphor Thr180), 1 : 200, PA5-104616, Invitrogen; YAP1, 1 : 100, ab205270, abcam; pYAP1 (phosphor S127), 1 : 100,ab76252, abcam; CD133, 1 : 100, ab216323, Abcam) and the corresponding secondary antibodies [20 (link)]. Semiquantitative results were obtained by using the German semiquantitative scoring method, as previously described [21 (link)].
The endogenous peroxidase activity was blocked by soaking the deparaffinized specimens in 3.3% H2O2. The specimens were then incubated with primary antibodies (MST2, 1 : 200, ab87322, abcam; pMST2 (phosphor Thr180), 1 : 200, PA5-104616, Invitrogen; YAP1, 1 : 100, ab205270, abcam; pYAP1 (phosphor S127), 1 : 100,ab76252, abcam; CD133, 1 : 100, ab216323, Abcam) and the corresponding secondary antibodies [20 (link)]. Semiquantitative results were obtained by using the German semiquantitative scoring method, as previously described [21 (link)].
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