Weighed brain tissue samples were homogenized in ultra-pure-guanidine isothiocyanate (Sigma-Aldrich, Cat# 50,983) using innuSPEED Lysis Tubes P in SpeedMill Plus (Analytik Jena, Germany, Cat# 845-CS-1020250). RNA was isolated using Pure Link RNA mini kit (Invitrogen, Cat# 12183018A) as recommended by the manufacturer. DNA contamination was removed using PureLink™ DNase Set (Invitrogen, Cat# 10,977,035). RNA purity and the concentrations were determined using a NanoDrop spectrophotometer (Epoch, BioTek) and total RNA was converted into cDNA using High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems, Cat# 4,368,814) according to supplier’s protocol. Quantitative PCR (qPCR) was performed using with the primers shown at Supplementary Table 1. AMPIGENE qPCR Green Mix Hi-ROX (Enzo Life Sciences, Cat# ENZ-NUC104) was used under the following conditions: 95 °C for 2 min for initial denaturation, followed by 5 s (40 cycles) at 95 °C and 30 s at 57 °C. Data were generated in the final step at 95 °C for 15 s and melting curves (65 to 95 °C) were acquired at the end for each primer set. Relative gene expression was calculated by 2−ΔΔCt method by normalizing gene expression to β-actin (Aktas et al. 2015 (link)). Data were generated for four mice per group and each biological replicate has four technical replicates.