Genetic Mouse Models for Intestinal Research

Animal experiments were approved by the National Animal Experiment Board of Finland. Mice were housed and monitored according to the Federation of European Laboratory Animal Science Associations guidelines and recommendations. The mice were weighed and their health was closely monitored during the experiment. The mouse lines Lef1^fl/fl (25 (link)), Apc^fl/fl (76 (link)), Lgr5-EGFP-IRES-Cre^ERT2 (4 (link)), Rosa26^LSL-TdTomato (Jackson Laboratory, stock no. 021875), Apc^Min/+ (Jackson Laboratory, stock no. 002020), Villin-Cre^ERT2 (Jackson Laboratory, stock no. 020282), and Villin-Cre (Jackson Laboratory, stock no. 021504) have been described previously. Lef1^fl/fl mice with mixed C57BL/6 and 129SV background were used after backcrossing to the C57BL/6 strain for >6 generations. All experiments were performed three times with independent cohorts. Approximately equal numbers of male and female mice of same age were used for all the experiments.

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Heino S., Fang S., Lähde M., Högström J., Nassiri S., Campbell A., Flanagan D., Raven A., Hodder M., Nasreddin N., Xue H.H., Delorenzi M., Leedham S., Petrova T.V., Sansom O, & Alitalo K. (2021). Lef1 restricts ectopic crypt formation and tumor cell growth in intestinal adenomas. Science Advances, 7(47), eabj0512.

Publication 2021

Lgr5-EGFP-IRES-CreERT2 Rosa26LSL-TdTomato ApcMin/+ Villin-CreERT2 Villin-Cre

C57bl mice European Female Ires Male Mice Strain Tdtomato Villin

Corresponding Organization : Wihuri Research Institute

Other organizations : China Pharmaceutical University, SIB Swiss Institute of Bioinformatics, Cancer Research UK, Cancer Research UK Scotland Institute, Wellcome Centre for Human Genetics, University of Oxford, Hackensack University Medical Center, Center for Discovery, University of Lausanne, Ludwig Cancer Research

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Variable analysis

independent variables

Mouse lines: Lef1^(fl/fl), Apc^(fl/fl), Lgr5-EGFP-IRES-Cre^(ERT2), Rosa26^(LSL-TdTomato), Apc^(Min/+), Villin-Cre^(ERT2), and Villin-Cre

dependent variables

Mice weight
Mouse health

control variables

Mice were housed and monitored according to the Federation of European Laboratory Animal Science Associations guidelines and recommendations
Approximately equal numbers of male and female mice of same age were used for all the experiments

controls

Positive controls: Not specified
Negative controls: Not specified

Annotations

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