The Aedes aegypti strain used in the experiments was a genetically diverse strain (GDS1) collected as eggs at 12 sites along the Pacific coast of Chiapas State, Mexico. The genetically diverse Ae. albopictus strain (GDS2) was also collected from four sites along the Pacific coast of Chiapas State [18 (link)].
Larvae were reared at a density of 1.5 insects/mL in 61 × 41 × 7.5 cm3 plastic trays containing 2000 mL of dechlorinated water and were fed with liquid Laboratory Rodent Diet (LabDiet, Fort Worth, TX, USA), as described previously [23 (link)]. Pupae were separated by sex as a function of body size using a plate separator (John W. Hock, Model 5412, Gainesville, FL, USA) and confirmed by examination of the genital lobe using a Stemi 508 stereomicroscope (Carl Zeiss, Oberkochen, Germany). Both colonies were maintained under controlled conditions at 28 ± 2 °C for larvae and 26 ± 2 °C with 80 ± 5% relative humidity (RH) for adults, and a photoperiod of 14:10 h (light:darkness (L:D)) for both stages.
Larvae were reared at a density of 1.5 insects/mL in 61 × 41 × 7.5 cm3 plastic trays containing 2000 mL of dechlorinated water and were fed with liquid Laboratory Rodent Diet (LabDiet, Fort Worth, TX, USA), as described previously [23 (link)]. Pupae were separated by sex as a function of body size using a plate separator (John W. Hock, Model 5412, Gainesville, FL, USA) and confirmed by examination of the genital lobe using a Stemi 508 stereomicroscope (Carl Zeiss, Oberkochen, Germany). Both colonies were maintained under controlled conditions at 28 ± 2 °C for larvae and 26 ± 2 °C with 80 ± 5% relative humidity (RH) for adults, and a photoperiod of 14:10 h (light:darkness (L:D)) for both stages.
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