Inflammation Marker Analysis in Blood

Peripheral blood samples were collected for analysis of inflammation markers, before and after 8 weeks of intervention. The procedures performed for labeling CD3+ (anti-CD3 FITC) and CD4+ (anti-CD4 PerCP-CY5.5) as well as for labeling the specific monoclonal antibodies for the cytokines of interest [anti-interleukin (IL)-8 PE, IL-13 APC, IL-17 PE, IL-6 APC, IL-2 APC, IL-10 PE, tumor necrosis factor (TNF) APC] were previously described.^{33 (link)} All reagents used in the analyses were from Becton Dickinson, San Diego, CA.

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Uzeloto J.S., de Toledo-Arruda A.C., Silva B.S., Braz A.M., de Lima F.F., Grigoletto I., Ramos D., Golim M.A, & Ramos E.M. (2022). Effect of physical training on cytokine expression in CD4+ T lymphocytes in subjects with stable COPD. Therapeutic Advances in Respiratory Disease, 16, 17534666221091179.

Publication 2022

anti-CD3 FITC anti-CD4 PerCP-CY5.5

Anti cd3 Blood Cy5 5 Cytokines Fitc Inflammation Interleukin 10 Interleukin 13 Interleukin 17 Interleukin 2 Interleukin 6 Interleukin 8 Monoclonal antibodies Tumor necrosis factor

Corresponding Organization :

Other organizations : Universidade Estadual Paulista (Unesp), Universidade Federal Fluminense

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Variable analysis

independent variables

8 weeks of intervention

dependent variables

Inflammation markers

control variables

Peripheral blood samples collected before and after intervention

controls

Positive control: not specified
Negative control: not specified

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