Micro-CT was performed on the left femora and 3rd lumbar vertebrae (LV3) of each mouse following overnight fixation with 10% neutral buffered formalin. μCT was completed as previously described (Lee et al., 2017 (link); Philbrick et al., 2015 (link)). Briefly, femora were scanned using a Scanco μCT40 scanner (Scanco Medical AG, Basserdorf, Switzerland) at a voxel size of 12 × 12 × 12 μm (55 kVp x-ray voltage, 145 μA intensity, and 200 ms integration time). Total femora (cancellous + cortical bone) were evaluated followed by analysis of cortical bone in the mid femur diaphysis and cancellous bone in the distal femur metaphysis. For the femoral diaphysis, 20 consecutive slices (240 μm) of bone were evaluated and cross-sectional volume (cortical and marrow volume, mm3), cortical volume (mm3), marrow volume (mm3), and cortical thickness (μm) were measured. For the femoral metaphysis, 42 consecutive slices (504 μm) of cancellous bone, and 75 slices (1,050 μm) proximal to the growth plate, were evaluated. For the vertebra, the region of interest was located between the cranial and caudal growth plates (151 ± 2 slices, 1,812 ± 24 μm). Direct cancellous bone measurements included cancellous bone volume fraction (bone volume/tissue volume, BV/TV, %), connectivity density (mm−3), trabecular number (mm−1), trabecular thickness (μm) and trabecular spacing (μm).