Enrichment of miR-526b-3p in U87 and U251 cells

Probes, including biotinylated miR-526b-3p WT and biotinylated miR-526b-3p Mut, or control probes purchased from RiboBio (Guangzhou, China) were transfected into U87 and U251 cells. Then, DNaseI was added to RNA solution, and incubation was for 5 min at 65°C. Afterwards, streptavidin-coated magnetic beads (New England BioLabs, USA) were incubated for another 4 h. Finally, the beads were washed with PBS and extracted RNA. MiR-526b-3p enrichment was detected via RT-qPCR analysis [19 (link)].

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Qiu L., Xing S., Lang F, & Bao Q. (2022). MiR-526b-3p Inhibits the Resistance of Glioma Cells to Adriamycin by Targeting MAPRE1. Journal of Oncology, 2022, 2402212.

Publication 2022

control probes streptavidin-coated magnetic beads

Cells Streptavidin

Corresponding Organization : Xuzhou Medical College

Other organizations : Central Hospital of Zibo, Xintai People's Hospital

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Variable analysis

independent variables

Probes, including biotinylated miR-526b-3p WT and biotinylated miR-526b-3p Mut

dependent variables

MiR-526b-3p enrichment detected via RT-qPCR analysis

control variables

Control probes purchased from RiboBio (Guangzhou, China)

controls

Positive control: Not explicitly mentioned.
Negative control: Control probes purchased from RiboBio (Guangzhou, China)

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