Spectrophotometric and Chromatographic Analysis

UV–Vis double beam spectrophotometer Halo DB-20S (Dynamica GmbH, Dietikon, Switzerland) was used for all spectrophotometric measurements. The chemical and reagents used for the evaluation of phenolic contents and antioxidant activity were obtained from Alfa Aesar (Karlsruhe, Germany) and Sigma Aldrich (Steinheim, Germany). The solvents (methanol, acetonitrile, and formic acid) and reference standards of polyphenols (purity greater than 95%) for UHPLC analyses were purchased from Sigma Aldrich (Steinheim, Germany). All materials for antimicrobial activity tests (Nutrient agar—NA, Sabouraud dextrose agar—SDA, Müller–Hinton broth—MHB, and Sabouraud dextrose broth—SDB) were obtained from Torlak Institute of Virology, Vaccines and Sera (Belgrade, Serbia). Regarding the anti-inflammatory activity assays, the reagents and assay kits were purchased as follows: arachidonic acid, purified prostaglandin H synthase (PGHS)-1 from ram seminal vesicles, human recombinant COX-2, and NS-398 were purchased from Cayman Chemical Co. (Ann Arbor, MI, USA), Na₂EDTA and tris(hydroxymethyl)-aminomethanhydro-chlorid (Titriplex III) from Merck KGaA (Darmstadt, Germany). Hematin and indomethacin (porcine) were obtained from ICN (Aurora, OH, USA), competitive PGE2 EIA kit from Enzo Life Sciences Inc. (Farmingdale, NY, USA), formic acid and DMSO (>99.98% purity) from Sigma-Aldrich (St. Louis, MO, USA), and epinephrine hydrogen tartarate from Fluka (St. Louis, MO, USA).