Fibroblasts were stained with the fluorescent dye rhodamine 123, plated and imaged as previously described.13 (link) Raw images were binarised and mitochondrial morphological characteristics were quantified. These were length or aspect ratio (AR, the ratio between the major and minor axis of the ellipse equivalent to the mitochondrion), degree of branching or form factor (FF, defined as (Pm2)/(4πAm), where Pm is the length of mitochondrial outline and Am is the area of mitochondrion), and number of mitochondria per cell (Nc).
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Quantitative Mitochondrial Morphology Analysis
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Other organizations : University of Sheffield, National Institute on Aging, National Institutes of Health, Radboud University Nijmegen, Radboud University Medical Center, University College London, National Hospital for Neurology and Neurosurgery, Sheffield Children's Hospital
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Variable analysis
dependent variables
- Mitochondrial length or aspect ratio (AR)
- Degree of branching or form factor (FF)
- Number of mitochondria per cell (Nc)
- Fibroblasts were stained with the fluorescent dye rhodamine 123
- Fibroblasts were plated and imaged as previously described in the referenced study (13)
- No positive or negative controls were explicitly mentioned in the provided information.
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