Enzyme Inhibition Assays for Plant Extracts

The elastase inhibitory activity of the prioritized extract was evaluated using elastase from porcine pancreas (PPE) type IV (Merck KGaA, Darmstadt, Germany) and the substrate N-succinyl-Ala-Ala-Ala-p-nitroanilide (Merck KGaA, Darmstadt, Germany), as previously described [36 (link)]. The capacity of the extract to inhibit the catalytic action of tyrosinase in the oxidation of L-DOPA to dopachrome was determined, as previously reported [37 (link)].

Free full text: Click here

Louka X.P., Sklirou A.D., Le Goff G., Lopes P., Papanagnou E.D., Manola M.S., Benayahu Y., Ouazzani J, & Trougakos I.P. (2021). Isolation of an Extract from the Soft Coral Symbiotic Microorganism Salinispora arenicola Exerting Cytoprotective and Anti-Aging Effects. Current Issues in Molecular Biology, 44(1), 14-30.

Publication 2021

N-succinyl-Ala-Ala-Ala-p-nitroanilide

Ala ala ala Catalytic action Dopachrome Elastase Elastase iv Inhibit Inhibitory L dopa Pancreas Porcine Tyrosinase

Corresponding Organization : National and Kapodistrian University of Athens

Other organizations : Centre National de la Recherche Scientifique, Institut de Chimie des Substances Naturelles, Tel Aviv University

Top 5 similar protocols

Variable analysis

independent variables

Prioritized extract

dependent variables

Elastase inhibitory activity
Tyrosinase inhibitory activity

control variables

Elastase from porcine pancreas (PPE) type IV
Substrate N-succinyl-Ala-Ala-Ala-p-nitroanilide
L-DOPA

controls

Positive control: Not specified
Negative control: Not specified

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!