Quantitative Cytokine ELISA Protocol

Concentrations of IL-6, IL-12, and TNFα in culture supernatants or mouse sera were determined by quantitative ELISA using cytokine-specific coating Abs and biotinylated detection Abs (eBioscience) as previously described (10 (link), 22 (link)). Mouse serum levels of IL-10 and IL-1β were analyzed by ELISA using cytokine-specific coating Abs and biotinylated detection Abs (R&D) as previously described (10 (link), 22 (link)). Levels of IFN-β in mouse sera were measured using the VeriKine-HS^™ Mouse Interferon Beta Serum ELISA Kit (PBL Assay Science, Piscataway, NJ) following the manufacturer’s protocol.

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Lalani A.I., Moore C.R., Luo C., Kreider B.Z., Liu Y., Morse HC I.I.I, & Xie P. (2014). Myeloid cell TRAF3 regulates immune responses and inhibits inflammation and tumor development in mice. Journal of immunology (Baltimore, Md. : 1950), 194(1), 334-348.

Publication 2014

biotinylated detection Abs VeriKine-HS™ Mouse Interferon Beta Serum ELISA Kit

Assay Cytokine Elisa Il 10 Il 12 Il 1β Interferon beta Mouse Serum Tnfα

Corresponding Organization : Rutgers, The State University of New Jersey

Other organizations : National Institute of Allergy and Infectious Diseases, National Institutes of Health

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Variable analysis

independent variables

Not explicitly mentioned

dependent variables

Concentrations of IL-6, IL-12, and TNFα in culture supernatants or mouse sera
Levels of IL-10 and IL-1β in mouse sera
Levels of IFN-β in mouse sera

control variables

Not explicitly mentioned

controls

Cytokine-specific coating Abs and biotinylated detection Abs (eBioscience) were used for quantitative ELISA of IL-6, IL-12, and TNFα
Cytokine-specific coating Abs and biotinylated detection Abs (R&D) were used for ELISA of IL-10 and IL-1β
The VeriKine-HS™ Mouse Interferon Beta Serum ELISA Kit (PBL Assay Science) was used to measure levels of IFN-β in mouse sera

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