Immunostaining of Brain Tissue and Cells

Immunostaining of brain sections and dissociated cells were performed as described previously.^{66 (link), 67 (link)} The following primary antibodies were used: Chicken anti-GFP (Thermo Fisher Scientific Inc.), rabbit anti-GFP (Thermo Fisher Scientific Inc.), rabbit anti-RFP (Thermo Fisher Scientific Inc.), mouse anti-MAP2 (BioLegend, San Diego, CA, USA), guinea pig anti-vGlut (EMD Millipore, Billerica, MA, USA), rabbit anti-vGAT (PhosphoSolutions, Aurora, CO, USA), mouse anti-GAD6 (DSHB) and mouse anti-Sig-1 R (Santa Cruz Biotechnology, Inc., Dallas, TX, USA)). Appropriate secondary antibodies conjugated with Alexa Fluor dyes (Thermo Fisher Scientific Inc) were used to detect primary antibodies.