ATMi [36] (link), ATRi [35] (link), and Nu7026 [42] (link) were as previously described [30] (link) and used at 10, 5, and 10 µM, respectively. Olaparib [85] (link) (AZD2281) (Selleck Chemicals) was dissolved in DMSO to 3 mM and used at a final concentration of 3 µM. An equal concentration of DMSO solvent was used as a control vehicle for each drug. Hydroxyurea (MP Biochemicals) was dissolved in water at a final concentration of 1 M and used at 0.5 and 10 mM in complete DMEM for 18 and 2 h, respectively.
To treat cells with inhibitors during the final 28 h or 8 h of a 48 h SV40 infection, the media was removed from the cells at 20 and 40 hpi, respectively, and replaced with fresh media containing either inhibitor or DMSO. Exposure of cells to inhibitors during periods of a 48 h SV40 infection was as per [30] (link).
To treat cells with inhibitors during the final 28 h or 8 h of a 48 h SV40 infection, the media was removed from the cells at 20 and 40 hpi, respectively, and replaced with fresh media containing either inhibitor or DMSO. Exposure of cells to inhibitors during periods of a 48 h SV40 infection was as per [30] (link).
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