To account for any biological variability, a total of six units of fresh (no older than 42 days) human packed red blood cells (RBC) and 12 compatible units of human plasma were obtained from the local blood bank. Six units of plasma were combined to obtain an averaged plasma sample. The residual six units of plasma where mixed with the six units of compatible RBC to reconstitute whole blood.
Ferumoxytol was diluted in 30 mL sample tubes of saline (n=5), human plasma (n=5) and human whole blood (n=5) at five gradually increasing concentrations within the range typically used in clinical imaging (1/2048 [0.26 mM], 1/1024 [0.52 mM], 1/512 [1.05 mM], 1/256 [2.1 mM], 1/128 [4.2 mM]). The 15 sample tubes were sealed and then placed in an MR compatible water bath at 37°C for 20 minutes before imaging in the MR-scanner. To avoid settling of particles or cells, samples were inverted gently every 15–20 minutes.
Ferumoxytol was diluted in 30 mL sample tubes of saline (n=5), human plasma (n=5) and human whole blood (n=5) at five gradually increasing concentrations within the range typically used in clinical imaging (1/2048 [0.26 mM], 1/1024 [0.52 mM], 1/512 [1.05 mM], 1/256 [2.1 mM], 1/128 [4.2 mM]). The 15 sample tubes were sealed and then placed in an MR compatible water bath at 37°C for 20 minutes before imaging in the MR-scanner. To avoid settling of particles or cells, samples were inverted gently every 15–20 minutes.
