Immunoblotting of Pancreatic Tissue Samples

Pancreatic tissue samples from the various experimental groups were homogenized on ice with RIPA buffer (150 mM NaCl, 1% NP40, 50 mM Tris pH 8.0, 0.5% sodium deoxycholate and 0.1% SDS) supplemented with a cocktail of protease inhibitors (Roche); The homogenized tissue was then centrifuged and the cleared supernatant was subjected to immunoblotting applying anti-cathepsin L (1:1000, Santacruz biotechnology, sc-6498), anti-pSTAT3 (Santacruz biotechnology, Sc-8059), anti-STAT3 (1:1000, Santa Cruz biotechnology, Sc-7179), anti-pIκB (1:1000, Cell signaling Technology, #2859), anti-IκB (1:1000, Cell signaling Technology, #4812) or anti-actin (1:10000, Sigma Aldrich, A1978) antibodies, essentially as described^{58 (link), 69 (link), 70} .