Bacteria and bacterial DNA were prepared as described previously with minor modifications.26 (link),27 In brief, the bacterial DNA was isolated by the enzymatic lysis method using lysozyme (Sigma-Aldrich Co. LCC., Tokyo, Japan) and achromopeptidase (Wako). The DNA samples were purified by treatment with RNase A (Wako), followed by precipitation with 20% PEG solution (PEG6000 in 2.5 M NaCl). The DNA was pelleted by centrifugation, rinsed with 75% ethanol, and dissolved in TE buffer.
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Bacterial DNA Isolation Protocol
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Achromopeptidase
Bacteria
Bacterial dna
Buffer
Centrifugation
Enzymatic
Ethanol
Lysozyme
Nacl
Peg6000
Rnase
Corresponding Organization : Waseda University
Other organizations : Keio University, RIKEN Center for Integrative Medical Sciences, Toyohashi University of Technology, Okayama University
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Protocol cited in 16 other protocols
Variable analysis
independent variables
- Bacterial DNA preparation method
- Purified DNA samples
- Lysozyme (Sigma-Aldrich Co. LCC., Tokyo, Japan)
- Achromopeptidase (Wako)
- RNase A (Wako)
- PEG6000 in 2.5 M NaCl
- 75% ethanol
- TE buffer
- Positive control: Not explicitly mentioned
- Negative control: Not explicitly mentioned
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