CRISPR-Cas9 Plasmid Constructs

For a complete list of constructs, see Supplementary Table 1. pSpCas9(BB)-P2A-GFP (PX458) (Addgene plasmid # 48138)^{65 (link)}, PX551(Addgene plasmid # 60957)^{66 (link)}, and pX330-U6-Chimeric_BB-CBh-hSpCas9 (Addgene plasmid # 42230)^{65 (link),66 (link)} were a gift from Feng Zhang. CAPZB2-TwinStrepII was a gift from Peter Barr-Gillespie (Addgene plasmid # 83194). pAAV-FLEx-tdTomato was a gift from Edward Boyden (Addgene plasmid # 28306). All constructs generated in the Soderling lab were validated with sequencing (Eton).

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Spence E.F., Dube S., Uezu A., Locke M., Soderblom E.J, & Soderling S.H. (2019). In vivo proximity proteomics of nascent synapses reveals a novel regulator of cytoskeleton-mediated synaptic maturation. Nature Communications, 10, 386.

Publication 2019

PX551 pX330-U6-Chimeric_BB-CBh-hSpCas9 pAAV-FLEx-tdTomato

Chimeric Plasmid Tdtomato

Corresponding Organization :

Other organizations : Duke University, Duke University Hospital, Duke Medical Center

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Variable analysis

independent variables

PSpCas9(BB)-P2A-GFP (PX458) (Addgene plasmid # 48138)
PX551(Addgene plasmid # 60957)
PX330-U6-Chimeric_BB-CBh-hSpCas9 (Addgene plasmid # 42230)
CAPZB2-TwinStrepII (Addgene plasmid # 83194)
PAAV-FLEx-tdTomato (Addgene plasmid # 28306)

dependent variables

Not explicitly mentioned

control variables

Not explicitly mentioned

controls

Positive controls: Not specified
Negative controls: Not specified

Annotations

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