PBMC Isolation and Culture from COPD Patients

As described previously [9 (link), 10 (link)], PBMCs of COPD patients (administrated at the Second Affiliated Hospital of Xi’an Jiao Tong University, Xi’an, China) were collected via lymphocyte separation medium (Sigma, Shanghai, China). The resulting PBMCs were cultured in DMEM plus 10% FBS, and necessary supplements [30 (link)]. Experiments and protocols requiring human samples were approved by the Ethics Committee and Internal Review Board of Xi’an Jiao Tong University. Written-informed consent was provided by each patient.

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Li P., Li X., Wu Y., Li M, & Wang X. (2017). A novel AMPK activator hernandezine inhibits LPS-induced TNFα production. Oncotarget, 8(40), 67218-67226.

Publication 2017

lymphocyte separation medium

Copd Ethics committee Human Lymphocyte Patient Supplements

Corresponding Organization :

Other organizations : Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an Jiaotong University, First Hospital of Xi'an, Xi'an Medical University, First Affiliated Hospital of Xi'an Jiaotong University

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Variable analysis

independent variables

COPD patient PBMC samples

dependent variables

Not explicitly mentioned

control variables

Lymphocyte separation medium (Sigma, Shanghai, China)
DMEM plus 10% FBS, and necessary supplements
Experiments and protocols requiring human samples were approved by the Ethics Committee and Internal Review Board of Xi'an Jiao Tong University
Written-informed consent was provided by each patient

positive controls

Not specified

negative controls

Not specified

Annotations

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