Investigating Developmental Effects of Signaling Modulators

All analyses were performed at 36 hpf. Embryos were exposed to the following compounds in fish facility water from 10 hpf to 36 hpf17 (link)45 (link): 10 μM PGE2 (Sigma), 10 μM Indomethacin (Sigma); 0.5 μM Forskolin (Abcam, ab120058), 0.5 μM H89 (Abcam, ab120341), 20 μM Arachidonic acid (Sigma, A9673). DMSO carrier content was at 0.1%. Equivalent amount of DMSO was added to the media as control. For PGG2 (0.1 mg/ml, Cayman, 17010) and PGH2 (0.1 mg/ml, Santa Cruz, sc201266), embryos were injected with these chemicals in DMSO at the bud stage46 .

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Chen J., He J., Li L., Yang D, & Luo L. (2016). Cyp2aa9 regulates haematopoietic stem cell development in zebrafish. Scientific Reports, 6, 26608.

Publication 2016

Indomethacin Forskolin Arachidonic acid

Arachidonic acid Cayman Dmso Embryos Fish Forskolin Indomethacin Pge2 Pgg2 Pgh2

Corresponding Organization :

Other organizations : Southwest University, Chongqing Medical University, Stomatological Hospital of Chongqing Medical University

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Variable analysis

independent variables

10 μM PGE2
10 μM Indomethacin
0.5 μM Forskolin
0.5 μM H89
20 μM Arachidonic acid
0.1 mg/ml PGG2
0.1 mg/ml PGH2

dependent variables

Embryo responses at 36 hpf

control variables

Fish facility water
DMSO carrier content at 0.1%

controls

Positive control: Equivalent amount of DMSO added to the media
Negative control: Not explicitly mentioned

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