Apoptosis Assay for Primary Cells and Cell Lines

For primary cells derived from SD rats, single cell suspensions were prepared as mentioned in the “Isolation of lung type II pneumocytes cells” section. For cell lines, A549 cells were digested by trypsin without EDTA. The cell concentration was adjusted to 3 × 10 [5 (link)]/mL and stained with a 100ul binding solution containing 5ul annexin V-fluorescein isothiocyanate and 5ul PI solution in a 100ul binding solution. Apoptosis was assayed using a Novo Cyte flow cytometry (Acea Biosciences, San Diego, CA, USA) .

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Liang G.P., Xu J., Cao L.L., Zeng Y.H., Chen B.X., Yang J., Zhang Z.W, & Kang Y. (2019). Piezo1 induced apoptosis of type II pneumocytes during ARDS. Respiratory Research, 20, 118.

Publication 2019

Novo Cyte flow cytometry

A549 cells Annexin v Apoptosis Cell Cell lines Cells isolation Edta Flow cytometry Fluorescein Isothiocyanate Lung Rats Trypsin Type ii pneumocytes

Corresponding Organization :

Other organizations : Sichuan University, Chengdu Medical College

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Variable analysis

independent variables

Cell type (primary cells from SD rats vs. A549 cell line)

dependent variables

Apoptosis

control variables

Cell concentration (3 × 10^5/mL)
Binding solution composition (100 μL containing 5 μL annexin V-FITC and 5 μL PI)

controls

No explicit mention of positive or negative controls

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