Western Blot Analysis of Insulin Signaling Pathway

Western blots were performed as described previously [29 (link)] for glycogen synthase kinase 3 beta (GSK3β; Abcam, Cambridge, UK), phospho-GSK3β Ser9 (Abcam), glycogen synthase 1 (GS; Abcam), phospho-GS Ser641 (Abcam), β-actin (Abcam), protein kinase B (Akt; Cell Signaling Technology, Beverly, MA, USA), phospho-Akt Ser473 (CST), insulin receptor substrate 1 (IRS-1; CST), phospho-IRS-1 Ser307 (CST), phospho-IRS-1 Y632 (CST), forkhead box protein O1 (FoxO1; CST) and phospho-FoxO1 Thr24 (CST) [30 (link),31 (link)].

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Teng W., Li Y., Du M., Lei X., Xie S, & Ren F. (2019). Sulforaphane Prevents Hepatic Insulin Resistance by Blocking Serine Palmitoyltransferase 3-Mediated Ceramide Biosynthesis. Nutrients, 11(5), 1185.

Publication 2019

GSK3β β-actin protein kinase B (Akt;

Actin Forkhead box protein o1 Glycogen synthase 1 Gsk3β Irs 1 Protein kinase b Western blots

Corresponding Organization :

Other organizations : China Agricultural University, Washington State University, Cornell University

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

Glycogen synthase kinase 3 beta (GSK3β)
Phospho-GSK3β Ser9
Glycogen synthase 1 (GS)
Phospho-GS Ser641
Protein kinase B (Akt)
Phospho-Akt Ser473
Insulin receptor substrate 1 (IRS-1)
Phospho-IRS-1 Ser307
Phospho-IRS-1 Y632
Forkhead box protein O1 (FoxO1)
Phospho-FoxO1 Thr24

control variables

β-actin (used as a loading control)

controls

No positive or negative controls were explicitly mentioned.

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