Perfusion-Fixed Murine Brain Preparation for MRI

Brains were prepared according to a previously reported protocol (Gimenez et al., 2017 (link)). After transcardiac perfusion with a 4% paraformaldehyde solution containing Gd-chelate (6.25 mM of Gd-DOTA; Guerbet Laboratories, Roissy, France), mice were decapitated. After the removal of skin and muscles, the skull was immersed in the same fixing solution for 4 days and transferred into Fomblin oil (FenS chemicals, Goes, Netherlands), an oil that generates no MRI signal but has a magnetic susceptibility close to that of brain tissue. MRI was performed at least 7 days after brain fixation. This protocol ensures a homogeneous distribution of the Gd-DOTA throughout the whole mouse brain (Gimenez et al., 2016 ). The contrast agent decreased brain T1 from 1000 ± 102 to 110 ± 13 ms and brain T2 from 27.3 ± 3.1 to 18.3 ± 2.6 ms (data not shown), allowing a reduction in acquisition times.

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de Souza D.A., Mathieu H., Deloulme J.C, & Barbier E.L. (2023). Evaluation of kernel low-rank compressed sensing in preclinical diffusion magnetic resonance imaging. Frontiers in Neuroscience, 17, 1172830.

Publication 2023

Gd-DOTA

Brain Contrast agent Gd dota Mouse Muscles Paraformaldehyde Perfusion Skin Skull Susceptibility Tissue

Corresponding Organization :

Other organizations : Grenoble Institute of Neurosciences, Centre Hospitalier Universitaire de Grenoble

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Variable analysis

independent variables

Transcardiac perfusion with a 4% paraformaldehyde solution containing Gd-chelate (6.25 mM of Gd-DOTA; Guerbet Laboratories, Roissy, France)

dependent variables

Brain T1 (decreased from 1000 ± 102 to 110 ± 13 ms)
Brain T2 (decreased from 27.3 ± 3.1 to 18.3 ± 2.6 ms)

control variables

Mice were decapitated after transcardiac perfusion
Removal of skin and muscles
Skull was immersed in the same fixing solution for 4 days
Transferred into Fomblin oil (FenS chemicals, Goes, Netherlands), an oil that generates no MRI signal but has a magnetic susceptibility close to that of brain tissue
MRI was performed at least 7 days after brain fixation

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