The colon tissues
were cut into sections (each 5-μm thick) and then stained using
Masson’s trichrome staining following the established procedure.
After a short period of deparaffinization, the sections were submerged
in Bouin solution and left to incubate at 37 °C overnight. The
sections underwent a 2–3 min staining process using Celestine
blue after being rinsed with distilled water. Subsequently, the samples
were washed with water and colored using Mayer hematoxylin solution
for a duration of 2–3 min. After that, the sections underwent
another water wash and were differentiated with an acidic ethanol
solution for a brief period. Following a 10 min rinse with running
water, the sections were stained using Li Chunhong Acid Fuchsin Staining
solution, and 1% phosphomolybdic acid was used to differentiate the
sections for 10 min. After extraction from phosphomolybdic acid, the
samples underwent staining using aniline blue solution, cleansing
with distilled water, and a 2 min treatment with a mild acid solution.
Eventually, the slices underwent dehydration using 95% and 100% alcohol,
followed by sealing.
were cut into sections (each 5-μm thick) and then stained using
Masson’s trichrome staining following the established procedure.
After a short period of deparaffinization, the sections were submerged
in Bouin solution and left to incubate at 37 °C overnight. The
sections underwent a 2–3 min staining process using Celestine
blue after being rinsed with distilled water. Subsequently, the samples
were washed with water and colored using Mayer hematoxylin solution
for a duration of 2–3 min. After that, the sections underwent
another water wash and were differentiated with an acidic ethanol
solution for a brief period. Following a 10 min rinse with running
water, the sections were stained using Li Chunhong Acid Fuchsin Staining
solution, and 1% phosphomolybdic acid was used to differentiate the
sections for 10 min. After extraction from phosphomolybdic acid, the
samples underwent staining using aniline blue solution, cleansing
with distilled water, and a 2 min treatment with a mild acid solution.
Eventually, the slices underwent dehydration using 95% and 100% alcohol,
followed by sealing.
