Placental Oxidative Stress Evaluation

Placental tissue (200 mg) was homogenized. The reactive oxygen species (ROS) concentration analysis was performed by the chemiluminescence (CL) assay using luminol as an indicator of radical formation. The tissue samples were recorded at room temperature using an LB 940 luminometer (Berthold Technologies, Bad Wildbad, Germany) in the presence of enhancers as described previously [16 (link)]. The contents of malondialdehyde (MDA) were assayed using colorimetric methods with a spectrophotometer (Biomate 5, Thermo Electron Corporation, Rochester, NY, U.S.A.). Total antioxidant capacity (TAC) was measured with a commercial kit (Nanjing JianCheng Bioengineering Institute, A015, Nanjing, China) as per manufacturer’s instructions.