Ammonium acetate (≥98% purity, for
HPLC, acetic acid ammonium salt) and formic acid (≥98% purity)
were purchased from Acros Organics (Geel, Belgium). Methanol (for
LC) was purchased from Merck (Darmstadt, Germany). Ammonium hydroxide
solution (30–33% in water, w/w) was purchased from Sigma-Aldrich
(St. Louis, MO, USA). Ammonium acetate (10 mM) and formic acid (0.5%,
v/v) were prepared in 25% (v/v) methanol in deionized water. 225 μL
of ammonium hydroxide solution was mixed with 29.8 mL of 25% (v/v)
methanol in deionized water in order to achieve an approximate volumetric
concentration of 0.5% ammonium hydroxide.
HPLC, acetic acid ammonium salt) and formic acid (≥98% purity)
were purchased from Acros Organics (Geel, Belgium). Methanol (for
LC) was purchased from Merck (Darmstadt, Germany). Ammonium hydroxide
solution (30–33% in water, w/w) was purchased from Sigma-Aldrich
(St. Louis, MO, USA). Ammonium acetate (10 mM) and formic acid (0.5%,
v/v) were prepared in 25% (v/v) methanol in deionized water. 225 μL
of ammonium hydroxide solution was mixed with 29.8 mL of 25% (v/v)
methanol in deionized water in order to achieve an approximate volumetric
concentration of 0.5% ammonium hydroxide.
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