Insulin Tolerance Test in Fasted Mice

ITT was conducted in mice after fasting of 6 hours as described elsewhere ^{45 (link)}. At Time 0, blood glucose was measured to set a baseline. insulin (1 unit/kg ITT) was injected i.p., blood samples were collected from the tail vein at 15, 30, and 60 minutes. The plasma glucose was measured by a glucometer (FreeStyle Lite system, Abbott Diabetes Care CA, USA).

I n j e c t i o n v o l u m e (μ L) = m o u s e w e i g h t (k g) \times \frac{\frac{1 u n i t}{k g} i n s u l i n}{0.1 u n i t / m l} = m o u s e w e i g h t (k g) \times 10 m l / k g i n s u l i n = m o u s e w e i g h t (g) \times 10

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Wang L., Savani R., Bernabucci M., Lu Y., Singh I., Xu W., El Ouaamari A., Wheeler M.B., Grill H.J., Rossi M.A, & Pang Z.P. (2024). State-dependent central synaptic regulation by GLP-1 is essential for energy homeostasis. Research Square.

Publication Preprint 2024

FreeStyle Lite system

Corresponding Organization : Rutgers, The State University of New Jersey

Other organizations : The University of Texas Medical Branch at Galveston, University of Toronto, The University of Texas Southwestern Medical Center, New York Medical College, Child Health and Development Institute, Rutgers Sexual and Reproductive Health and Rights, University of Pennsylvania

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Variable analysis

independent variables

Insulin (1 unit/kg ITT) was injected i.p.

dependent variables

Blood glucose levels were measured at 0, 15, 30, and 60 minutes

control variables

Mice were fasted for 6 hours prior to the experiment
Blood glucose was measured at Time 0 to set a baseline

controls

No positive or negative controls were explicitly mentioned in the provided information.

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