This method describes
the procedure to determine the activity of an enzyme in TBU units
per gram of enzyme (TBU/g): To a 100 mL double-walled stirred reaction
vessel (kept at 40 °C) were added 20 mL of 25 mM Pi buffer at
pH 7.3 and 400 μL of TBU (2%) and the emulsion stirred for 2
min. Then an automatic titrator (Metrohm) using 0.1 M sodium hydroxide
was switched on, and the pH was adjusted to 7.5. Immobilized enzyme
(∼30 units maximum) was added, and after the first 100 μL,
base was added and the base consumption was monitored for 5 min. Unit
definition: 1 TBU unit = 1 μmol butyric acid released per min/g
enzyme.
the procedure to determine the activity of an enzyme in TBU units
per gram of enzyme (TBU/g): To a 100 mL double-walled stirred reaction
vessel (kept at 40 °C) were added 20 mL of 25 mM Pi buffer at
pH 7.3 and 400 μL of TBU (2%) and the emulsion stirred for 2
min. Then an automatic titrator (Metrohm) using 0.1 M sodium hydroxide
was switched on, and the pH was adjusted to 7.5. Immobilized enzyme
(∼30 units maximum) was added, and after the first 100 μL,
base was added and the base consumption was monitored for 5 min. Unit
definition: 1 TBU unit = 1 μmol butyric acid released per min/g
enzyme.
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