SIM was performed as previously described by Lund et al. (2018 (link)). In brief, coverslips (High-precision, 1.5H, 22 ± 22 mm, 170 ± 5 mm, Marienfeld) were sonicated in 1 M KOH for 15 min before being washed and then incubated in poly-L-Lysine solution for 30 min. Coverslips were then washed and dried before fixed cells (suspended in water) were dried onto coverslips and mounted with SlowFadeTM Gold antifade reagent (Thermo Fisher Scientific).
SIM was performed using a v4 DeltaVision OMX 3D-SIM system fitted with a Blaze module (Applied Precision, GE Healthcare, Issaquah, USA) with lasers used to illuminate samples. For each Z-slice (0.125 nm), images were taken in five phase shifts and three angles. To reconstruct images, the software Softworx (GE Healthcare, Issaquah, USA) was used with optimisation for a 1.516 immersion oil. The same software was used for deconvolution and image alignment.
SIM was performed using a v4 DeltaVision OMX 3D-SIM system fitted with a Blaze module (Applied Precision, GE Healthcare, Issaquah, USA) with lasers used to illuminate samples. For each Z-slice (0.125 nm), images were taken in five phase shifts and three angles. To reconstruct images, the software Softworx (GE Healthcare, Issaquah, USA) was used with optimisation for a 1.516 immersion oil. The same software was used for deconvolution and image alignment.
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