Targeting vectors for making Cxcl12DsRed and Cxcl12fl mice were generated by recombineering31 . Linearized targeting vector was electroporated into C57BL-derived Bruce4 ES cells. Correctly targeted clones were identified by Southern blotting. Following expansion, these ES cell clones were injected into C57BL/6-Tyrc-2J blastocysts. Chimeric mice were bred with C57BL/6-Tyrc-2J mice to obtain germline transmission. The Frt-flanked Neo selection cassette was removed by subsequent mating with Flpe mice32 . The resulting mice were backcrossed for at least three generations onto a C57BL/Ka background. Mice used in this study included Ubc-creER42 , CMV-cre43 , Vav1-cre44 , Nestin-cre45 , Tie2-cre46 , Lepr-cre47 , Prx1-cre26 (link), and LoxpEYFP48 (all from Jackson Laboratory). Scfgfp/+ mice were described previously10 (link). Col2.3-cre mice23 (link) were obtained from Drs. F. Liu and B. Kream (University of Connecticut). Col2.3-GFP mice49 were obtained from Dr. D. Rowe. Tamoxifen chow (Harlan) containing tamoxifen citrate (Spectrum Chemical) at 400mg/kg, with 5% sucrose added, was administrated to mice for 2–4 months to induce recombination by CreER.
All mice were housed in the Unit for Laboratory Animal Medicine at the University of Michigan or in the Animal Resource Center at the University of Texas Southwestern Medical Center. All protocols were approved by the University of Michigan Committee on the Use and Care of Animals and by the UT Southwestern Institutional Animal Care and Use Committee.
All mice were housed in the Unit for Laboratory Animal Medicine at the University of Michigan or in the Animal Resource Center at the University of Texas Southwestern Medical Center. All protocols were approved by the University of Michigan Committee on the Use and Care of Animals and by the UT Southwestern Institutional Animal Care and Use Committee.
