For transmission electron microscopy work, mouse skin tissue samples were cut to a minimal size (1 mm x 1mm) and fixed with 2 % glutaraldehyde/2 % paraformaldehyde in 0.1M cacodylate buffer, pH 7.4. Further processing was done according using standard protocols, including a number of optimized steps for skin tissue, as described previously40 (link). Resulting 60 nm ultrathin sections were analysed with a Tecnai 12-biotwin transmission electron microscope (ThermoFisher) and representative images were taken with a 2K CCD Veleta camera (EMSIS, Münster, Germany).
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Optimized TEM Protocol for Mouse Skin
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Corresponding Organization :
Other organizations : Université Libre de Bruxelles, Erasmus Hospital, Max Planck Institute for Molecular Biomedicine, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Ghent University, VIB-UGent Center for Medical Biotechnology, University of Helsinki, KU Leuven, Walloon Excellence in Lifesciences and Biotechnology
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- Tissue sample preparation method (cutting to minimal size, fixation with 2% glutaraldehyde/2% paraformaldehyde in 0.1M cacodylate buffer, pH 7.4, and further processing according to standard protocols with optimized steps for skin tissue)
- Ultrastructural features of mouse skin tissue observed using transmission electron microscopy
- Tissue type (mouse skin)
- Transmission electron microscope (Tecnai 12-biotwin)
- Camera (2K CCD Veleta)
- No positive or negative controls were explicitly mentioned in the provided information.
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